The Egg Grape Chambers were first employed by Dr. Eggman during Sonic the Hedgehog's year-long absence in space. When the city of Albion was attacked and subsequently destroyed, the vast majority of its citizens were captured and sent to the Egg Grape Chambers. Almost a year later, Eggman had his agent Hunter engage the Dark Legion and free echidnas following Sonic and the Chaotix's rescue of Knuckles' father Locke. Hunter managed to send numerous echidnas to the Egg Grape Chambers before being stopped by Super Knuckles.
The original batch of Egg Grape Chambers were lost when Ixis Naugus was freed from the Zone of Silence and the Egg Vineyard was pulled into the portal. However, Eggman soon had a batch of new Egg Grape Chambers ready, and successfully transported Ixis Naugus, Mammoth Mogul, the Destructix and the Arachne. The Destructix were liberated and employed by Dr. Finitevus while the Arachne were killed due to the effects of the Egg Grape Chambers. Mammoth Mogul managed to preserve himself with his Chaos powers, while Naugus became a victim of their effects, becoming a mindless beast. Both managed to escape when they were released by Anonymous.
During Eggman's successful attack on Knothole, the Egg Fleet and Snively's battle suit transported all but five of the Kingdom of Acorn's citizens to the Egg Grape Chambers. Only Charmy Bee became a victim of them however, losing some of his memory. However, a rescue effort by Sonic, Tails, Knuckles, Amy and NICOLE resulted in the Mobians freedom and transportation to New Mobotropolis. Following this, Eggman once again attempted to use a revamped Egg Grape Chamber to drain the revived Enerjak of his Chaos powers. While some of his power was drained, Enerjak managed to escape from the Egg Grape Chamber and completely destroy New Megaopolis and the Egg Fleet.
The known victims who died in the Egg Grape Chambers are Simon, Floren-Ca, Xenin, Rykor, The Council of Ants, Kanewisher, Bimmy, King Bee, Queen Bee, Princess Bee, members of The Golden Hive Colony and several members of the Dark Legion. Possible victims might include Gala-Na, Syntar, Yanar, Rita-Le and Gae-Na.